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JoVE Journal Neuroscience
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
 

Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis

Article DOI: 10.3791/61283-v • 14:51 min • September 27th, 2020
September 27th, 2020 •
1, 1, 1
1Department of Biology, Saint Mary's College of California

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This paper describes the isolation and culturing of embryonic rat sympathetic neurons from the superior cervical ganglia. It also provides detailed protocols for immunocytochemical staining and for preparing neuronal extracts for mass spectrometric analysis.

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Keywords: Rat Sympathetic Neurons Embryonic Superior Cervical Ganglia Morphological Analysis Proteomic Analysis Cell Culture Poly-D-lysine Dissection Medium Control Medium Enzymatic Digestion Centrifugation Institutional Animal Care And Use Committee
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