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JoVE Journal Genetics
A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
 

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Article DOI: 10.3791/61506-v • 10:39 min • September 17th, 2020
September 17th, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biology, William Paterson University

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Here is a protocol to identify genetic interactions through an increased copy number suppressor screen in Saccharomyces cerevisiae. This method allows researchers to identify, clone, and test suppressors in short-lived yeast mutants. We test the effect of the copy number increase of SIR2 on lifespan in an autophagy null mutant.

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Keywords: Suppressor Screen Genetic Links Chronological Lifespan Saccharomyces Cerevisiae Aging Dietary Restriction Hallmarks Of Aging Budding Yeast Autophagy Over-expression Suppressor Screen
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