Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Laser-Capture Microdissection RNA-Sequencing for…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
 

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Article DOI: 10.3791/61517-v • 08:33 min • August 5th, 2020
August 5th, 2020 •
1,2, 1,2, 1, 1,2,3, 1,2,3, 1,3
1Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

Capitoli

Riepilogo
Automatic Translation

Presented here is a protocol for laser-capture microdissection (LCM) of plant tissues. LCM is a microscopic technique for isolating areas of tissue in a contamination-free manner. The procedure includes tissue fixation, paraffin embedding, sectioning, LCM and RNA extraction. RNA is used in the downstream tissue-specific, temporally resolved analysis of transcriptomes.

Tags

Keywords: Laser-capture Microdissection RNA-sequencing Spatial Transcriptome Temporal Transcriptome Plant Cells Tissue Fixation Tissue Embedding Paraffin Embedding Barley Seed Vacuum Infiltration Tissue Processing Dehydration Xylene Paraffin Infiltration
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter