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Immunologia e infezioni

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小鼠B细胞发育的流式细胞学表征

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Flow Cytometric Characterization of Murine B Cell Development

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小鼠B细胞发育的流式细胞学表征

DOI:

10.3791/61565-v

•

08:25 min

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January 22, 2021

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Faith M. Harris*¹, Karoline A. Meagher*¹, Maggie Zhong, Benjamin J. Daniel, Mark Eckersdorff, Jesse A. Green, Vera Voronina, Chunguang Guo, Andre Limnander, Lynn E. Macdonald

¹Regeneron Tech Centers,Regeneron Pharmaceuticals, Inc., ²Regeneron Therapeutic Proteins,Regeneron Pharmaceuticals, Inc., ³Immunology and Inflammation,Regeneron Pharmaceuticals, Inc.

Capitoli

00:04Introduction
00:55Stain Cells and Prepare Compensation
05:30Flow Cytometric Data Acquisition and Analysis
06:08Results: Flow Cytometric Analysis for the Characterization of B Cells
07:24Conclusion

Summary

Traduzione automatica

我们在这里描述了通过流式细胞术对腹膜，脾脏和骨髓组织中小鼠免疫B细胞室异质性的简单分析。该协议可以适应并扩展到其他小鼠组织。

Tags

Keywords: Flow Cytometry B Cell Development Murine Models Autoimmunity Antibody Therapeutics Fluorophores Phenotyping Genetic Manipulation Bone Marrow Spleen Viability Dye Single Color Compensation

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