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无转基因生成血液衍生神经元细胞
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
GM-Free Generation of Blood-Derived Neuronal Cells
DOI:

08:11 min

February 13, 2021

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Capitoli

  • 00:04Introduction
  • 00:47Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
  • 01:25Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
  • 02:21Sorting of Newly Generated Dedifferentiated Cells
  • 03:33Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
  • 04:10Culturing of Neuronal Differentiated Blood Cells
  • 04:44Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
  • 06:08Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
  • 07:41Conclusion

Summary

Traduzione automatica

我们提出了一种无转基因(无转基因)的方法,从重新编程的周围血细胞中获取具有神经元表型的细胞。激活与新型人类GPI相关蛋白质相关的信号通路,揭示了获得人类多能干细胞的高效无转基因方法。

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