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1Université Côte d’Azur,Inserm UMR1065, C3M, Team “Cellular and Molecular Pathophysiology of Obesity”, Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine,Karolinska Institutet, Stockholm, Sweden, 3Université Côte d’Azur,Inserm UMR1065, C3M, Team “Haematometabolism in Diseases”, Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS),Université de Toulouse, CNRS, UPS, Toulouse, France
Capitoli
- 00:04Introduction
- 00:35Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation
- 02:59Mouse and Human White Adipose Tissue Staining
- 05:28Mouse and Human White Adipose Tissue Clearing
- 06:173D Confocal Imaging
- 07:233D Adipose Tissue Image Quantitative Result Extraction
- 07:57Results: Representative Adipose Tissue Labeling and Analysis
- 09:34Conclusion
Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.
