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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
 

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Article DOI: 10.3791/61669-v • 05:28 min • September 10th, 2020
September 10th, 2020 •
1, 1, 1
1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw

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The protocol presented here is intended to demonstrate the occurrence of heterologous interactions between Golgi-resident type III membrane proteins with cytoplasmically exposed N- and/or C-termini in live mammalian cells using the most recent variant of the split luciferase complementation assay.

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Keywords: Heterologous Complexes Golgi-resident Type III Membrane Proteins Split Luciferase Complementation Assay HEK293 T-cells Transfection Luminescence Nucleotide Sugar Transporters SLC35A2 SLC35A3 Protein-protein Interactions
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