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Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity
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Capitoli
- 00:04Introduction
- 00:41Transcardial Perfusion
- 01:59Brain Extraction
- 04:44Local Field Potential (LFP) Spontaneous Activity Recording
- 06:04Results: Representative Spontaneous Sharp-Wave Ripple (SWR) Recordings
- 07:17Conclusion
This protocol describes the preparation of horizontal hippocampal-entorhinal cortex (HEC) slices from mice exhibiting spontaneous sharp-wave ripple activity. Slices are incubated in a simplified interface holding chamber and recordings are performed under submerged conditions with fast-flowing artificial cerebrospinal fluid to promote tissue oxygenation and the spontaneous emergence of network-level activity.
