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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
 

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Article DOI: 10.3791/61732-v • 07:33 min • May 26th, 2021
May 26th, 2021 •
1,2, 1,2, 1,2
1Department of Physiology, Louisiana State University Health Sciences Center - New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center, Louisiana State University Health Sciences Center - New Orleans

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In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

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Adipose-derived Stem Cells Rhesus Macaque Isolation Proliferation Differentiation Metabolic Assays Insulin-stimulated Glucose Uptake Lipogenesis Lipolysis Collagenase Digestion RBC Lysis Cell Culture Tissue Processing
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