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In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
 

In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model

Article DOI: 10.3791/61837-v • 08:22 min • October 27th, 2020
October 27th, 2020 •
*1, *1, 1, 1, 1, 1, 1
1Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University
* These authors contributed equally

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The distinct effects of different degrees of hypothermia on myocardial protection have not been thoroughly evaluated. The goal of the present study was to quantify the levels of cell death following different hypothermia treatments in a human cardiomyocyte-based model, laying the foundation for future in-depth molecular research.

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Keywords: In Vitro Myocardial Protection Hypothermia-preconditioning Human Cardiac Myocytes Cell Death CCK-8 Assay Apoptosis Flow Cytometry Annexin V-FITC Propidium Iodide
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