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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
 

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Article DOI: 10.3791/61870-v • 10:59 min • May 28th, 2021
May 28th, 2021 •
1, 1, 1, 1,2,3
1Huffington Center on Aging, Baylor College of Medicine, 2Department of Molecular and Human Genetics, Baylor College of Medicine, 3Howard Hughes Medical Institute, Baylor College of Medicine

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Stimulated Raman scattering (SRS) microscopy allows selective, label-free imaging of specific chemical moieties and it has been effectively employed to image lipid molecules in vivo. Here, we provide a brief introduction to the principle of SRS microscopy and describe methods for its use in imaging lipid storage in Caenorhabditis elegans.

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Stimulated Raman Scattering Microscopy Label-free Imaging Lipid Storage Dynamics Caenorhabditis Elegans Chemical Imaging Technology Quantitative Detection Of Lipids Live Animals Photo Bleaching Fluorescence Staining Genetic Tools Biochemical Tools Model Organisms Regulators Of Lipid Biology And Metabolism SRS Microscope Laser Beams Periscope Picosecond Light Source Exit Infrared Laser Input Port Scanner Of The Microscope Laser Control Software Pump Laser Power Pump Signal Beam Expander Microscope Objectives Relay Mirrors
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