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Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans
 

Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Article DOI: 10.3791/64092-v • 07:25 min • December 9th, 2022
December 9th, 2022 •
*1,2, 2,3,4, 2,4,5, *2
1Graduate Program in Developmental Biology, Baylor College of Medicine, 2Huffington Center on Aging, Baylor College of Medicine, 3Integrative Program of Molecular and Biochemical Sciences, Baylor College of Medicine, 4Department of Molecular and Human Genetics, Baylor College of Medicine, 5Howard Hughes Medical Institute, Baylor College of Medicine
* These authors contributed equally

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The present protocol describes lipid supplementation methods in liquid and on-plate cultures for Caenorhabditis elegans, coupled with longitudinal studies and gene transcriptional analysis from bulk or a few worms and worm tissues.

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Keywords: Lipid Supplementation Longevity Gene Transcriptional Analysis Caenorhabditis Elegans OP50 Bacteria Bacterial Dilution Dietary Restriction BDR Medium Lipid Stock Solution Liquid Culture Synchronized Caenorrhabditis Elegans NGM Agar Plate Transcriptional Changes Tissue Dissection Liquid Feeding Hydro Techniques
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