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Immunology and Infection

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JoVE Journal Immunology and Infection
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
 

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Article DOI: 10.3791/61895-v • 07:46 min • April 30th, 2021
April 30th, 2021 •
1, 1, 1, 2, 3, 3, 2, 3,4, 1
1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute

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In the age of immunotherapy and single-cell genomic profiling, cancer biology requires novel in vitro and computational tools for investigating the tumor-immune interface in a proper spatiotemporal context. We describe protocols to exploit tumor-immune microfluidic co-cultures in 2D and 3D settings, compatible with dynamic, multiparametric monitoring of cellular functions.

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Microfluidic Co-culture Models Immune Response In Vitro Tumor Microenvironments 2D Co-cultures 3D Co-cultures Tumor-immune Cell Crosstalks Anti-cancer Treatments Immune Cell Recruitment Patient-derived Samples State-of-the-art Microscopies Francesco Noto PhD Student Department Of Oncology And Molecular Medicine Istituto Superiore Di Sanita Nicoleta Manduca Esther Maccafeo PhD Students Universita Cattolica Del Sacro Cuore Department Of Translational Medicine And Surgery Plasma Cleaner Reactive Iron-etching Machine Clean Room Murine-spleen Cells Tumor Cell Line Cell Suspensions Growth Medium
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