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Journal / Bioengineering / Simple, Affordable, and Modular Patterning of…
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Bioengineering

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JoVE Journal Bioengineering
Simple, Affordable, and Modular Patterning of Cells using DNA
 

Simple, Affordable, and Modular Patterning of Cells using DNA

Article DOI: 10.3791/61937-v • 08:59 min • February 24th, 2021
February 24th, 2021 •
1, 2, 1, 3, 4,5,6, 7,8, 9, 2,6,10
1Graduate Program in Bioengineering, University of California San Francisco and University of California Berkeley, 2Department of Pharmaceutical Chemistry, University of California San Francisco, 3Scribe Biosciences, 4Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 5California Institute for Quantitative Biosciences, University of California San Francisco, 6Chan Zuckerberg Biohub, University of California San Francisco, 7Department of Chemical & Biomolecular Engineering, University of California Berkeley, 8Helen Wills Neuroscience Institute, University of California Berkeley, 9Department of Mechanical Engineering, University of California Berkeley, 10Center for Cellular Construction, University of California San Francisco

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Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

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Keywords: Cell Patterning DNACMOD Pack 2D/3D Cell Culture Cell-cell Interactions Morphogenesis Easy Adoption Photo Lithography Amine Modified Oligos Reductive Amination Universal Anchor Universal Co-anchor Cell Suspension
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