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Capitoli
- 00:04Introduction
- 00:30Microstructured Single Cell Array Fabrication
- 03:16Cell Seeding
- 04:21Perfusion System
- 05:13Time-Lapse Microscopy
- 06:44Fluorescent Marker mRNA Transfection
- 07:34Image Analysis and Fluorescence Readout
- 08:29Results: Representative Image Analysis and Fluorescence Readout
- 09:34Conclusion
We present a method for the acquisition of fluorescence reporter time courses from single cells using micropatterned arrays. The protocol describes the preparation of single-cell arrays, the setup and operation of live-cell scanning time-lapse microscopy and an open-source image analysis tool for automated preselection, visual control and tracking of cell-integrated fluorescence time courses per adhesion site.
