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JoVE Journal Medicine
Characterization of a Novel Human Organotypic Retinal Culture Technique
 

Characterization of a Novel Human Organotypic Retinal Culture Technique

Article DOI: 10.3791/62046-v • 05:51 min • June 9th, 2021
June 9th, 2021 •
*1, 1, 1, *1
1Buchanan Ocular Therapeutics Unit, Department of Ophthalmology and the New Zealand National Eye Centre, University of Auckland
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera.

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Keywords: Organotypic Retinal Culture Retinal Explant Retinal Integrity Retinal Diseases Drug Testing Tissue Handling Dulbecco's Modified Eagle Medium Antibiotics Antimycotics Dissection Sample Collection Sclera Optic Nerve Head
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