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鸡卵外胚胎和脉络膜尿囊血管的制备作为体内模型，用于对比增强超声成像和微泡介导的药物递送研究
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies
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鸡卵外胚胎和脉络膜尿囊血管的制备作为体内模型，用于对比增强超声成像和微泡介导的药物递送研究

DOI:
10.3791/62076-v

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09:53 min

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February 09, 2021

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, , , ,
1Department of Biomedical Engineering, Thoraxcenter,Erasmus MC University Medical Center Rotterdam, 2Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences,Delft University of Technology

Capitoli

  • 00:05Introduction
  • 00:43Embryo Preparation
  • 05:24Removing Embryo and CAM from Yolk for Microscopy Imaging
  • 07:25Results: Representative In Vivo Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery
  • 09:06Conclusion

Summary

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该协议描述了有关如何获得和使用5至8天大的鸡胚胎及其脉络膜尿囊膜（CAM）作为体内模型的三种方法，以研究对比增强超声成像和微泡介导的药物递送。

Tags

Keywords: Chicken EmbryoEx OvoChorioallantoic MembraneContrast-enhanced UltrasoundMicrobubble-mediated Drug DeliveryIn Vivo ModelEgg IncubationEggshell OpeningAlbumin RemovalEmbryo VisualizationYolk Exposure

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