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JoVE Journal
Immunology and Infection
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Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
 

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury

Article DOI: 10.3791/62097-v 14:48 min March 21st, 2021
March 21st, 2021

Capitoli

Riepilogo

Combined ozone and bacterial endotoxin exposed mice show wide-spread cell death, including that of neutrophils. We observed cellular adaptations such as disruption of cytoskeletal lamellipodia, increased cellular expression of complex V ATP synthase subunit β and angiostatin in broncho-alveolar lavage, suppression of the lung immune response and delayed neutrophil recruitment.

Tags

Lung Inflammation Acute Lung Injury Ozone Exposure LPS Instillation Bronchoalveolar Lavage Fluid (BAL) Lung Vascular Perfusate (LVP) Lung Cryosections Peripheral Blood Bone Marrow Mouse Model Imaging Fluorescence Microscopy
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