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A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
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Neuroscienze
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JoVE Journal Neuroscienze
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

DOI:
10.3791/62127-v

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07:56 min

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August 11, 2021

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, , ,
1Department of Medicine, School of Medicine,Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences,City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics,University of Florida College of Medicine

Capitoli

  • 00:04Introduction
  • 00:44Acute Rat Brain Slice Preparation
  • 02:01Ex Vivo Endogenous Monoamine Release Induction
  • 04:42HPLC Analysis
  • 05:29Results: Representative Endogenous Monoamine Release in Acute Brain Slices
  • 07:21Conclusion

Summary

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This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Tags

Endogenous Monoamine ReleaseBrain SlicesPlate-based AssayDorsal StriatumHippocampusEfflux BufferPharmacological ConditionsVesicular ReleaseTransporter-mediated Release

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