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Journal / Medicine / 小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养…
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小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养
 
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小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养

Article DOI: 10.3791/62236-v • 08:43 min • May 7th, 2021
May 7th, 2021 •
1,2,3, 1, 1,3, 1, 1,2,3, 1,2,3, 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

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这里介绍的方案提供了一种从小鼠心脏的窦房结（SAN）和房室结（AVN）区域分离心脏驻留巨噬细胞的分步方法。

Tags

医学，第171期，心脏传导系统，窦房结，房室结，常驻心脏巨噬细胞，流式细胞术，磁性活化细胞分选，MACS，荧光激活细胞分选，显微切割，原代细胞培养
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