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Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
 

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Article DOI: 10.3791/62319-v • 05:57 min • February 25th, 2021
February 25th, 2021 •
1,2, 3,4, 1, 1, 3, 1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center

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This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.

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Keywords: Glycosaminoglycans Polyacrylamide Gel Electrophoresis Silver Staining Glycocalyx Polysaccharide Endothelial Epithelial Resolving Gel Stacking Gel Ammonium Persulfate TEMED Sample Loading Buffer HS Oligosaccharide Ladder
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