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Präparation von Lamellen aus glasartigen biologischen Proben mit einem Zweistrahl-Rasterelektronenmikroskop für die Kryo-Elektronentomographie
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1Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science,Birkbeck College, University of London, 3Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory,Stanford University
Capitoli
- 00:04Introduction
- 00:48Screening Plunge Frozen Grids and Preparing for FIB-Milling
- 01:28On-Grid FIB Milling of Plunge Frozen Cells
- 04:18Results: Analysis of Cryo-ET Data from FIB-Milled Lamella
- 06:28Conclusion
