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神经节苷脂提取、纯化和分析
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JoVE Journal Biochimica
Ganglioside Extraction, Purification and Profiling
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神经节苷脂提取、纯化和分析

DOI:
10.3791/62385-v

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10:05 min

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March 12, 2021

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, ,
1Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 2Department of Neuroscience,Johns Hopkins University School of Medicine

Capitoli

  • 00:04Introduction
  • 01:36Small Scale Ganglioside Extraction and Partial Purification
  • 03:35Large Scale Ganglioside Extraction and Purification
  • 05:52Ganglioside Resolution and Staining
  • 08:02Results: Small and Large Scale Ganglioside Extraction and Partial Purification
  • 09:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

神经节苷脂是含唾液酸的鞘糖脂，在大脑中特别丰富。它们的两性性质需要有机/水提取和纯化技术，以确保最佳的回收率和准确的分析。本文概述了分析和制备规模的神经节苷脂提取，纯化和薄层色谱分析。

Tags

Keywords: Ganglioside ExtractionGanglioside PurificationGanglioside ProfilingCell Surface LipidsNerve Cell LipidsCell SignalingCell-cell RecognitionPolar Lipid AnalysisReverse-phase Cartridge ChromatographySolvent ExtractionPartitioningSolid-phase Extraction

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