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使用荧光激活的细胞核分选从新鲜冷冻的皮质中分离出成年人类星形胶质细胞群
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
DOI:

08:18 min

April 16, 2021

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Capitoli

  • 00:04Introduction
  • 00:42Frozen Tissue Dissociation and Ultracentrifugation
  • 02:13Fluorescence-Activated Nuclei Sorting (FANS)
  • 04:32Downstream Molecular Analyses
  • 05:58Results: Analysis of Human Astrocyte Populations after FANS
  • 07:40Conclusion

Summary

Traduzione automatica

我们开发了一种从新鲜冷冻组织中富集和分离人类星形胶质细胞群的方法,用于下游分子分析。

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