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Journal / Biology / Establishing Stable Binary Cultures of Symbiotic…
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Biology

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JoVE Journal Biology
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
 

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Article DOI: 10.3791/62484-v • 07:32 min • April 13th, 2021
April 13th, 2021 •
*1,2, *1,2, 1,2
1The Forsyth Institute, 2Harvard School of Dental Medicine
* These authors contributed equally

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We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria.

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Saccharibacteria Oral Cavity Obligate Parasites Co-culture Isolation Binary Co-culture Microbiological Laboratories Strains Devices Equipment Culturing Isolates Species Candidate-followed Radiation Species Actinobacterial Hosts Andrew Collins Postdoctoral Fellow Cultures Arachnia Propionica Tryptic Soy Broth Yeast Extract Incubate Filter Holders Track Etched 0.2 Micrometer Filter Membranes Foil Sterilize Autoclaving
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