Rivista
/
Biologia
/
In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

DOI:
10.3791/62494-v

•

08:15 min

•

September 08, 2021

•

, , ,
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Capitoli

  • 00:04Introduction
  • 00:44Bone Marrow Collection and Fixation
  • 01:30Embedding Bone Marrow in Agarose
  • 02:11Embedding Bone Marrow in Resin
  • 03:38Ultrathin Sectioning
  • 04:24Toluidine Blue Staining
  • 04:54Heavy Metal Staining
  • 05:31Transmission Electron Microscopy (TEM)
  • 06:02Results: In Situ Ultrastructure Exploration of Murine Megakaryopoiesis
  • 07:33Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to analyze ultrastructure of the megakaryocytes in situ using transmission electron microscopy (TEM). Murine bone marrows are collected, fixed, embedded in epoxy resin and cut in ultrathin sections. After contrast staining, the bone marrow is observed under a TEM microscope at 120 kV.

Tags

MegakaryocytesBone MarrowTransmission Electron MicroscopyIn Situ ExplorationMurineMaturation StagesUltrastructureFixationEmbeddingResin EmbeddingDehydrationStaining

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/62494?article_type=v

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
check_url/it/62494?article_type=v

Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
check_url/it/62494?article_type=v

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
check_url/it/62494?article_type=v

Rapid Analysis and Exploration of Fluorescence Microscopy Images
check_url/it/62494?article_type=v

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
check_url/it/62494?article_type=v

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
check_url/it/62494?article_type=v

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
check_url/it/62494?article_type=v

Miniaturized Sample Preparation for Transmission Electron Microscopy
check_url/it/62494?article_type=v

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging
check_url/it/62494?article_type=v

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Read Article