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Isolation of Mouse Megakaryocyte Progenitors

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Mouse Megakaryocyte Progenitors

Isolation of Mouse Megakaryocyte Progenitors

DOI:

10.3791/62498-v

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10:30 min

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May 20, 2021

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Quentin Kimmerlin, Manuela Tavian, Christian Gachet, François Lanza, Nathalie Brouard

¹Université de Strasbourg, INSERM UMR S1255, EFS Grand-EST, ²UMR-S1113 –IRFAC, Université de Strasbourg

Capitoli

00:04Introduction
00:38Mouse Bone Collection
03:10Magnetic Depletion of Lineage Positive Cells
07:17Results: Purification of MouseMEP and MKp from Femurs, Tibias, and Pelvic Bones by Flow Cytometry
09:50Conclusion

Summary

Traduzione automatica

This method describes the purification by flow cytometry of MEP and MKp from mice femurs, tibias, and pelvic bones.

Tags

Keywords: Mouse Megakaryocyte Progenitors Cell Sorting MEP MKP Bone C57 Black Six Iliac Crest Femur Tibia PBS NBCS Dissection Cell Isolation

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