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Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing
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Capitoli
- 00:04Introduction
- 00:45Tissue Clearing
- 01:57Preparing and Mounting the Cleared Tissue
- 04:19Imaging Cleared Tissue Samples
- 06:07Results: Imaging and Quantification of Neuronal Dendrites
- 07:22Conclusion
Neuronal dendritic morphology often underlies function. Indeed, many disease processes that affect the development of neurons manifest with a morphological phenotype. This protocol describes a simple and powerful method for analyzing intact dendritic arbors and their associated spines.
