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Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
 

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Article DOI: 10.3791/62594-v • 06:22 min • July 8th, 2021
July 8th, 2021 •
1,2, 3, 1,4, 5, 2, 1,2, 1
1Laboratory of Cell Biology, Department of Orthopedic Surgery, University Hospital of Tübingen, 2Department of Orthopedic Surgery, University Hospital of Tübingen, 3Institute for Bioinformatics and Medical Informatics, Faculty of science of the University of Tübingen, 4Medical faculty of the University of Tübingen, 5Bavarian Health and Food Safety Authority

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We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.

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Optical Sectioning Intervertebral Disc Visualization Embryonic Development Degeneration Collagen Type One Network Microscopy Chondrocytes DMEM Penicillin-Streptomycin Amphotericin B Freezing Thawing Bovine Disc Tissue Motion Segment Surgical Blade Cartilage Bovine Embryos
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