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体外重建人视网膜母细胞瘤
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JoVE Journal Ricerca sul cancro
Reconstruct Human Retinoblastoma In Vitro
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体外重建人视网膜母细胞瘤

DOI:
10.3791/62629-v

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06:52 min

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October 11, 2022

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1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

Capitoli

  • 00:04Introduction
  • 00:46Generation of Human Retinoblastoma
  • 04:39Results: In Vitro Reconstruction of Human Retinoblastoma
  • 06:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了一种通过在人胚胎干细胞（hESC）中引入双等位基因RB1突变来产生人视网膜母细胞瘤（RB）的方法。RB细胞系也可以在培养皿中使用分离的RB成功培养。

Tags

Keywords: RetinoblastomaIn VitroHuman Embryonic Stem CellsRB1 KnockoutRetinal Cell DifferentiationDispase BufferGrowth Factor Reduced Basement Membrane MatrixMedium 1Cyst Formation

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