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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
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Biologia
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JoVE Journal Biologia
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

DOI:
10.3791/62718-v

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06:06 min

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July 22, 2021

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, , ,
1Bone and Joint Center,Henry Ford Health System, 2Department of Orthopedic Surgery,Henry Ford Health System, 3Center for Molecular Medicine and Genetics,Wayne State University

Capitoli

  • 00:05Introduction
  • 00:56Sample Processing
  • 01:54Hard Tissue Homogenization
  • 03:05Soft Tissue Homogenization
  • 04:20Results: Tissue Identification and RNA Quality Assessment
  • 05:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

Tags

Keywords: RNA ExtractionOsteoarthritisHuman Knee JointGene ExpressionHistological AnalysisCartilageBoneMeniscusInfrapatellar Fat PadAnterior Cruciate LigamentSynoviumVastus Medialis Oblique MuscleLiquid NitrogenHomogenizationMortar And PestleAcid Guanidinium Phenol Solution

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