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从人骨关节炎膝关节收集和RNA提取
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Biologia
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JoVE Journal Biologia
Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
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从人骨关节炎膝关节收集和RNA提取

DOI:
10.3791/62718-v

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06:06 min

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July 22, 2021

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, , ,
1Bone and Joint Center,Henry Ford Health System, 2Department of Orthopedic Surgery,Henry Ford Health System, 3Center for Molecular Medicine and Genetics,Wayne State University

Capitoli

  • 00:05Introduction
  • 00:56Sample Processing
  • 01:54Hard Tissue Homogenization
  • 03:05Soft Tissue Homogenization
  • 04:20Results: Tissue Identification and RNA Quality Assessment
  • 05:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

从全膝关节置换术后的患者获得的原代组织为骨关节炎研究提供了一个实验模型，具有最大的临床可转化性。该协议描述了如何从七个独特的膝关节组织中鉴定，处理和分离RNA，以支持人类骨关节炎的机制研究。

Tags

RNA ExtractionOsteoarthritisHuman Knee JointGene ExpressionHistological AnalysisCartilageBoneMeniscusInfrapatellar Fat PadAnterior Cruciate LigamentSynoviumVastus Medialis Oblique MuscleLiquid NitrogenHomogenizationMortar And PestleAcid Guanidinium Phenol Solution

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