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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
 

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Article DOI: 10.3791/62791-v • 14:34 min • December 25th, 2021
December 25th, 2021 •
*1, *1, 1, 1, 1, 1, 2, 1
1Interdisciplinary Centre for Plant Genomics, Department of Plant Molecular Biology, University of Delhi South Campus, 2University School of Biotechnology, Guru Gobind Singh Indraprastha University
* These authors contributed equally

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Here we present, a method to visualize ternary complex formation between three protein partners using fluorescent-tagged proteins by BiFC based FRET-FLIM assay. This method is valuable for studying protein-protein interaction complexes in vivo.

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Keywords: Protein-protein Interactions BiFC FRET FLIM Tripartite Interaction MADS-box Transcription Factor Calcium Sensor Protein Homodimerization Nicotiana Benthamiana Agroinfiltration
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