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Mesoscopic Optical Imaging of Whole Mouse Heart
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Biologia
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JoVE Journal Biologia
Mesoscopic Optical Imaging of Whole Mouse Heart

Mesoscopic Optical Imaging of Whole Mouse Heart

DOI:
10.3791/62795-v

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08:53 min

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October 14, 2021

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, , , , , , , ,
1European Laboratory for Non-Linear Spectroscopy, 2Department of Biology,University of Florence, 3National Institute of Optics, National Research Council, 4Department of Physics and Astronomy,University of Florence, 5Department of Neurosciences, Psychology, Drugs and Child Health,University of Florence, 6Institute for Experimental Cardiovascular Medicine, Faculty of Medicine,University of Freiburg

Capitoli

  • 00:04Introduction
  • 00:40Heart Clearing
  • 02:48Cellular Membrane Staining
  • 04:18Mounting and Acquisition
  • 06:48Results: Mesoscopic Optical Imaging of Whole Mouse Heart
  • 08:15Conclusion

Summary

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We report a method for mesoscopic reconstruction of the whole mouse heart by combining new advancements in tissue transformation and staining with the development of an axially scanned light-sheet microscope.

Tags

Mesoscopic Optical ImagingWhole Mouse HeartClearing ProtocolMesos PIM TechnologyMicrometric ResolutionMouse HeartHydrogel SolutionClearing SolutionWheat Germ Agglutinin Alexa Fluor 633

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