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Extracellulaire vesikelopnametest via confocale microscoopbeeldvormingsanalyse
 
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Extracellulaire vesikelopnametest via confocale microscoopbeeldvormingsanalyse

Article DOI: 10.3791/62836-v • 08:32 min • February 14th, 2022
February 14th, 2022 •
*1,2, *1, 1,3, 2,4, 1, 2,4, 1
1The Brady Urological Institute, Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter, Institute for Basic Science (IBS)
* These authors contributed equally

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Extracellulaire blaasjes (EV's) dragen bij aan cellulaire biologie en intercellulaire communicatie. Er is behoefte aan praktische testen om de opname van EV's door de cellen te visualiseren en te kwantificeren. Het huidige protocol stelt de EV-opnametest voor door gebruik te maken van driedimensionale fluorescentiebeeldvorming via confocale microscopie, na EV-isolatie door een op nanofiltratie gebaseerd microfluïdisch apparaat.

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Biologie Nummer 180
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