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Flytande kromatografi i kombination med brytningsindex eller masspektrometrisk detektion för metabolitprofilering i lysatbaserade cellfria system
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Capitoli
- 00:04Introduction
- 01:08Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification
- 03:18Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition
- 04:54Extracting and Analyzing Data Post-Run
- 07:20Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification
- 09:20Setting Up a Run Sequence and Starting the LC-MS/MS Run
- 10:16Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data
- 11:45Results: Metabolite Profiling in Lysate-based Cell-free Systems
- 13:54Conclusion
