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Bioengineering

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JoVE Journal Bioengineering
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
 

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Article DOI: 10.3791/62852-v • 14:42 min • September 23rd, 2021
September 23rd, 2021 •
1,3, 2,3, 3, 3
1Graduate School of Genome Science & Technology, University of Tennessee Knoxville, 2Bredesen Center for Interdisciplinary Research, University of Tennessee Knoxville, 3Biosciences Division, Oak Ridge National Laboratory

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The protocols describe high-performance liquid chromatography methods coupled to refractive index or mass spectrometric detection for studying metabolic reactions in complex lysate-based cell-free systems.

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Liquid Chromatography Refractive Index Detection Mass Spectrometry Metabolite Profiling Lysate-based Cell-free Systems Carbon Metabolism Central Metabolites Quantitatively Describe Chemical Reactions Low Concentrations Complex Lysate Background Biosciences Division Graduate Researchers 1.5-milliliter Microcentrifuge Tubes Total Lysate Protein Cell-free Metabolic Engineering (CFME) Final Volumes Triplicate Per Time Point Terminate Reactions 5% Trichloroacetic Acid Sterile Water
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