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Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
 

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Article DOI: 10.3791/62882-v • 09:42 min • September 28th, 2021
September 28th, 2021 •
1, 1, 1, 1, 2, 2,3, 1
1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University

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Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.

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Keywords: Entomopathogenic Fungi Soil Samples Insect Pests Isolation Virulence Pathogenicity Screening Tenebrio Molitor Sabouraud Dextrose Agar Thermotolerance Conidia Production
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