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拟南芥种子表面灭菌的高通量、稳健性和高时间灵活性方法

JoVE Journal
Biologia

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JoVE Journal Biologia

High-throughput, Robust and Highly Time-flexible Method for Surface Sterilization of Arabidopsis Seeds

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拟南芥种子表面灭菌的高通量、稳健性和高时间灵活性方法

DOI:

10.3791/62893-v

•

07:28 min

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October 04, 2021

•

Mingai Li, Jiamei Yu, Enrico Barbaro, Claudio Varotto

¹Department of Biodiversity and Molecular Ecology, Research and Innovation Centre,Fondazione Edmund Mach

Capitoli

00:04Introduction
00:46Reagents and Media Preparation
01:59Aspirator Setup
02:37High-Throughput Liquid Surface Sterilization of Seeds
04:56Plating and Scoring of Arabidopsis on Half Strength Murashige and Skoog Plates
05:32Results: Germination Analysis of Arabidopsis Seeds After Surface Sterilization with the New Method
06:57Conclusion

Summary

Traduzione automatica

提供用于 拟南芥（Arabidopsis） 种子表面灭菌的高通量方案，通过带有真空泵的简单抽吸装置优化液体处理步骤。数百个种子样品可以在一天内进行表面灭菌。

Tags

Keywords: Arabidopsis Seed Surface Sterilization High-throughput Robust Time-flexible Functional Genomics Model Plant Species Ethanol Bleach Murashige And Skoog Medium Agar Kanamycin Aspirator Vacuum Pump Decantation Bottle Polyethylene Tube Aquarium Valve

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