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体外 通过微有机电荷调制场效应晶体管阵列进行多参数蜂窝分析
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
DOI:

10:05 min

September 20, 2021

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Capitoli

  • 00:04Introduction
  • 01:12Substrate Selection, Preparation, and Floating Gates (FG): Titanium Deposition
  • 02:11FG Patterning and Gate Dielectric Deposition
  • 03:55Opening of the Sensing Areas of the OCMFET for Electrical Activity Recording and Formation of the Vias to Access the Back of the FGs
  • 04:46Self-Alignment of Source and Drain with the FG
  • 05:15Gold Deposition, Channel Formation, and Patterning of the Sources, Drains, and Control Gates
  • 06:50Deposition and Activation of the Parylene C for pH Sensing
  • 07:57Semiconductor Deposition, Culturing Chamber Placing, and Final Cut-Out of the Device from the PET
  • 08:22Results: Cellular Electrical and Metabolic Activity Recordings with Micro OCMFET Arrays (MOA)
  • 09:27Conclusion

Summary

Traduzione automatica

在这里,我们提出了一种基于有机电荷调制场效应晶体管(OCMFET)的器件的制造方案,用于 体外 细胞接口。该器件称为micro OCMFET阵列,是一种灵活,低成本且无参考的器件,可以监测电活性细胞培养物的电和代谢活性活性。

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