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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
 

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Article DOI: 10.3791/62909-v • 12:15 min • February 8th, 2022
February 8th, 2022 •
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1Centre de recherche Cardio-Thoracique de Bordeaux, Univ. Bordeaux, 2IHU Liryc, Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Univ., 3Electrophysiology and Ablation Unit, Bordeaux University Hospital (CHU)

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Here, we present a protocol to obtain high resolution micro computed tomography images of healthy and pathological large mammalian whole hearts with collagen-selective contrast enhancement.

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Keywords: Micro Computed Tomography Cardiac Models Chronic Disease Tissue Preparation Contrast Enhancement Large Mammalian Microstructure Fibrosis Arrhythmia Cardioplegic Solution PBS/EDTA PMA Contrast Agent Coronary Osteo PTFE Tubing
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