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Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
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JoVE Journal Bioingegneria
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

DOI:
10.3791/62944-v

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10:51 min

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October 13, 2021

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, , , ,
1Department of Physiology and Biomedical Engineering,Mayo Clinic

Capitoli

  • 00:04Introduction
  • 01:31Solution Preparation
  • 04:21Experimental Setup
  • 07:05hPSC Culture and Analysis in Microcapsules
  • 08:31Results: Analysis of Characterization of Microcapsules and Encapsulation of hPSCs
  • 09:47Conclusion

Summary

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This article describes encapsulation of human pluripotent stem cells (hPSCs) using a co-axial flow focusing device. We demonstrate that this microfluidic encapsulation technology enables efficient formation of hPSC spheroids.

Tags

Keywords: Microfluidic FabricationCore-shell MicrocapsulesHuman Pluripotent Stem Cell Spheroids3D Cell CultureStem Cell EncapsulationScalable Stem Cell CultivationShear ProtectionCost-effective Cellular TherapyPEGDensity Gradient MediaMineral OilSurfactantDTTTEAPF127Cross-linker EmulsionShielding OilPEG4 Maleimide

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