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携带人多能干细胞球体的核壳微胶囊的微流体制造

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Bioingegneria
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JoVE Journal Bioingegneria

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

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携带人多能干细胞球体的核壳微胶囊的微流体制造

DOI:

10.3791/62944-v

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10:51 min

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October 13, 2021

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Kihak Gwon*¹, Hye Jin Hong*¹, Alan M. Gonzalez-Suarez, Gulnaz Stybayeva, Alexander Revzin

¹Department of Physiology and Biomedical Engineering,Mayo Clinic

Capitoli

00:04Introduction
01:31Solution Preparation
04:21Experimental Setup
07:05hPSC Culture and Analysis in Microcapsules
08:31Results: Analysis of Characterization of Microcapsules and Encapsulation of hPSCs
09:47Conclusion

Summary

Traduzione automatica

本文介绍了使用同轴流聚焦装置封装人多能干细胞（hPSC）。我们证明，这种微流体封装技术能够有效地形成hPSC球体。

Tags

Keywords: Microfluidic Fabrication Core-shell Microcapsules Human Pluripotent Stem Cell Spheroids 3D Cell Culture Stem Cell Encapsulation Scalable Stem Cell Cultivation Shear Protection Cost-effective Cellular Therapy PEG Density Gradient Media Mineral Oil Surfactant DTT TEA PF127 Cross-linker Emulsion Shielding Oil PEG4 Maleimide

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