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JoVE Journal Bioengineering
Microtubule Plus-End Dynamics Visualisering i Huntingtons sykdomsmodell basert på humane primære hudfibroblaster
 
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Microtubule Plus-End Dynamics Visualisering i Huntingtons sykdomsmodell basert på humane primære hudfibroblaster

Article DOI: 10.3791/62963-v • 10:38 min • January 8th, 2022
January 8th, 2022 •
*1,2, *2,3, 1,2, 3,4, 3,4, 1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
* These authors contributed equally

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Denne protokollen er dedikert til mikrotubule plus-end visualisering av EB3 proteintransfeksjon for å studere deres dynamiske egenskaper i primærcellekulturen. Protokollen ble implementert på humane primære hudfibroblaster hentet fra pasienter med Huntington sykdom.

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Bioingeniør Utgave 179 mikrotubuler transfeksjon EB3 primærkultur Huntingtons sykdom livstidsmikroskopi
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