Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Live-Cell Imaging of Transcriptional Activity at…
JoVE Journal
Biology

Questo Contenuto è ad Accesso gratuito.

JoVE Journal Biology
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
 

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Article DOI: 10.3791/62968-v • 09:07 min • September 20th, 2021
September 20th, 2021 •
1, 1, 1, 1
1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa

Capitoli

Riepilogo
Automatic Translation

This protocol presents a new reporter gene system and the experimental setup to detect transcription at DNA double-strand breaks with single-molecule sensitivity.

Tags

Keywords: Live-cell Imaging Transcriptional Activity DNA Double-strand Breaks Transcription Detection Single-cell Analysis DNA Damage Response Transcription-replication Crosstalk Doxycycline Induction Calibration Measurements Microcentrifuge Tube Preparation Transfection Glass Bottom Dish Microscopy Observation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter