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Bioengineering

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JoVE Journal Bioengineering
A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
 

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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This protocol details an enhanced method for synthesizing high yields of recombinant proteins from a Streptomyces venezuelae cell-free transcription-translation (TX-TL) system.

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Keywords: Streptomyces Venezuelae Cell-free Transcription-translation High GC Content Metabolic Enzymes Biosynthetic Pathways Heterologous Expression S30A Buffer S30B Buffer Dithiothreitol (DTT) Sonication Crude Extracts Non-model Organism Synthetic Biology Natural Product Applications
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