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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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JoVE Journal Bioingegneria
The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

DOI:
10.3791/63103-v

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10:34 min

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January 07, 2022

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, , , , ,
1The Department of Biomedical Engineering,Rutgers, The State University of New Jersey, 2The Department of Mechanical and Aerospace Engineering,Rutgers, The State University of New Jersey

Capitoli

  • 00:04Introduction
  • 00:43Device Fabrication‐Photolithography
  • 03:12Device Fabrication
  • 05:21Cell Preparation, Experimental Setup, Operation, and Analysis
  • 08:24Results: Cell Membrane Permeabilization and Electro‐Transfection Efficiency Using the Continuous Flow, Micro‐Electroporation System
  • 09:36Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the microfabrication techniques required to build a lab-on-a-chip, microfluidic electroporation device. The experimental setup performs controlled, single-cell-level transfections in a continuous flow and can be extended to higher throughputs with population-based control. An analysis is provided showcasing the ability to electrically monitor the degree of cell membrane permeabilization in real-time.

Tags

Micro-electroporationMicrofluidic Actuation DeviceCell Membrane PermeabilizationSU-8 PhotoresistGlass SlidePhoto MaskUV ExposureHard BakeProfilometryMF-319 Developer

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