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Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
 

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Article DOI: 10.3791/63155-v • 09:56 min • October 13th, 2021
October 13th, 2021 •
1,2,3, 1, 1,4, 5, 1, 1,3, 1,3,4
1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health, Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic

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Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research.

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Keywords: Extracellular Vesicles Escherichia Coli Bacterial Culture Scalable Isolation Purification Centrifugation Filtration Ultracentrifugation Therapeutics Preclinical Applications
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