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JoVE Journal Biochemistry
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
 

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Article DOI: 10.3791/63164-v • 14:39 min • April 22nd, 2022
April 22nd, 2022 •
1, 1, 1, 1, 1
1Department of Chemical Biological Sciences, Universidad Autónoma de Ciudad Juárez

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The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method.

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Keywords: Semi-targeted Ultra-high-performance Chromatography Mass Spectrometry Phenolic Metabolites Plasma Elderly Adults UPLC-MS/MS Protein Precipitation Metabolite Identification Brosimum Alicastrum Bioavailability Biotransformation Untargeted Analysis Semi-targeted Analysis Molecular Features Mass-to-charge Ratio Isotopic Distribution Fragmentation Pattern Polyphenol Metabolites
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