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Capitoli
- 00:04Introduction
- 00:35Drosophila Larvae Collection
- 01:23Culture Media and Tool Preparation
- 02:03Dissections and Brain Cultures
- 02:57Proliferation Assay, Brain Fixation, and Antibody Staining
- 04:24Results: Confocal Imaging of Stained Brain Cells
- 05:19Conclusion
A method to reactivate quiescent neural stem cells in cultured Drosophila brain explants has been established. Using this method, the role of systemic signals can be uncoupled from tissue-intrinsic signals in the regulation of neural stem cell quiescence, entry and exit.
